Part:BBa_K277060

3L.3_23.B3.08

3L.3_23.B3.08 is 748 bases long and is cloned into the pGem-T vector.

3L.3_23.B3.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B3.08 is a constituent of 3L.3_23.B3 (along with 3L.3_23.B3.01, 3L.3_23.B3.02, 3L.3_23.B3.03, 3L.3_23.B3.04, 3L.3_23.B3.05, 3L.3_23.B3.06, 3L.3_23.B3.07, and 3L.3_23.B3.09.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

mutation_affecting_coding_sequence YCL039W_re_remove_MmeI (480..491) removal of MmeI

gene YCL039W (-945..+1292) Protein of unknown function%2C involved in proteasome-dependent catabolite inactivation of fructose-1%2C6-bisphosphatase%3B contains six WD40 repeats%3B computational analysis suggests that Gid7p and Moh1p have similar functions

reverse_primer YCL039W_tagr1v1 (383..407)

Sequence (the first 748 bases correspond to coordinates 43964..44711 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 410
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 410
  Illegal NheI site found at 381
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 410
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 410
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 410
  
• 1000
  COMPATIBLE WITH RFC[1000]